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KMID : 0903520090520040315
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
2009 Volume.52 No. 4 p.315 ~ p.320
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Cloning and Characterization of the UDP Glucose/Galactose Epimerases of Oryza sativa
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Kim Seong-Kyong
Ahn Joong-Hoon
Kim Bong-Gyu
Kim Dae-Hwan
Hong Byoung-Seok
Jeon Young-Min
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Abstract
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UDP-glucose/galactose-4-epimerase (UGE) catalyzes the interconversion of UDP-glucose and UDP-galactose. Oryza sativa contains four UGEs. Expression of OsUGE3 was higher than other OsUGEs in all tissues except the stem. OsUGE1 expression was enhanced after drought, salt or UVirradiation stress. Four rice UGEs (OsUGE1-4) were cloned by polymerase chain reaction and sequenced. OsUGE1-4 were expressed in Escherichia coli with glutathione S-transferase fusion protein and purified. All four OsUGEs could covert UDP-glucose into UDP-galactose better than vice versa. Kinetic parameters of OsUGEs showed that OsUGE1 was most efficient. These OsUGEs could be used for biosynthesis of various UDP-sugars, which serve as a cosubstrates of UDP-dependent glycosyltransferase modification reactions of antibiotics or flavonoids.
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KEYWORD
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Oryza sativa, UDP-glucose/galactose-4-epimerase, UDP-sugar
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